![]() Using this powerful tool, it makes it possible to dissociate contribution from the mitochondrial genes vs that of the nuclear genes. A cybrid is then a hybrid cell which mixes the nuclear genes from one cell with the mitochondrial genes from another cell. The rho-zero cells do retain mitochondria and can grow in rich culture medium with certain supplements. Rho-zero cells are cells which have been depleted of their own mitochondrial DNA by prolonged incubation with ethidium bromide, a chemical which inhibits mitochondrial DNA replication. A special case of cybrid formation involves the use of rho-zero cells as the whole cell partner in the fusion. ![]() This enucleation can be effected by simultaneous application of centrifugal force and treatment of the cell with an agent that disrupts the cytoskeleton. Cytoplasmic hybrid A cytoplasmic hybrid is a eukaryotic cell line produced by the fusion of a whole cell with a cytoplast.
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